The North London based side must pick up all three points tonight and need a favour from Bayern Munich if they are to sustain any hope of advancing to the Last 16.
Özil starts in midfield alongside Santi Cazorla, Alexis Sanchez, Joel Campbell and Flamini.
The 27-year-old broke the assist record he created against West Brom last weekend when he setup Olivier Giroud early on in the first half.
The Germany international has now provided an assist in seven consecutive games which is a Premier League record.
Özil has a total of 11 assists in 12 games for the Gunners now which is the highest in Europe’s top five leagues including England, Spain, France, Italy and Germany.
Arsenal v Dinamo Zagreb
Kick-Off: 19:45 (GMT)
Stadium: Emirates Stadium
Arsenal confirmed startin line-up: Cech, Bellerin, Koscielny, Mertesacker, Monreal, Flamini, Cazorla, Campbell, Ozil, Alexis, Giroud
Subs: Ospina, Debuchy, Gabriel, Gibbs, Chambers, Ramsey, Reine-Adelaide
Dinamo Zagreb confirmed startin line-up: Eduardo, Matel, Taravel, Signall, Musa, Antollc, Rog, Pjaca, Machando, Fernandes, Hodzic