Barcelona go into the clash with a 2-1 aggregate lead but Atleti did manage to score an important away goal in the first-leg.
Barca boss Luis Enrique has decided to rest Arda Turan against his former side but the bearded winger is available on the bench if needed.
Turan has two goals and four assists in all competitions for Barca this season.
The midfield trio will instead be comprised of Rakitic, Sergio and Andres Iniesta while the MNS – Messi, Neymar and Suarez – will start upfront.
Atletico Madrid v Barcelona
Kick-Off: 19:45 (GMT)
Stadium: Vicente Calderón
Atletico Madrid confirmed starting line-up: Oblak, Juanfran, Godín, Lucas, Filipe Luís, Augusto, Saúl, Koke, Gabi, Carrasco, Griezmann
Barcelona confirmed starting line-up: 1.Ter Stegen 3. Piqué 4.Rakitic 5.Sergio 6.Alves 8.Iniesta 9.Suárez 10.Messi 11.Neymar Jr 14.Mascherano 18.Alba
Subs: 13. Bravo 2.Douglas 7.Arda 15.Bartra 17.Munir 20.Sergi Roberto 21. Adriano