The reigning La Liga champions beat City 4-1 at the Camp Nou but will have a few players out for their second encounter with the Premier League leaders.
Barca will be without three star players in Jordi Alba who has a hamstring injury, Iniesta who is sidelined for 6-8 weeks and Pique who suffered a blow to his ankle.
In other Barcelona team news head coach Luis Enrique will be without Aleix Vidal but Arda Turan has recovered from his ankle injury and was included in the match day squad.
Turan missed the 1-0 victory over Granada over the weekend and could be set to start in the role Iniesta occupies.
The midfield trio is set to be comprised of Rakitic, Busquets and either Turan or Rafinha.
The MSN – Messi, Neymar and Suarez – are fit and will almost certainly lead the attack for Barca at the Etihad.