The Lions are currently without a manager after sacking Hamza Hamzaoğlu last week despite the former Turkish national team head coach winning three trophies within six months.
Mustafa Denizli is expected to replace Hamzaoğlu but is unlikely to be officially appointed before the game against Atletico Madrid.
Galatasaray must beat Atleti and hope Benfica drop points to increase their chances of booking a place in the Champions League Last 16.
Full Galatasaray squad:
Fernando Muslera, Eray İşcan, Cenk Gönen – Aurelien Chedjou, Hakan Balta, Semih Kaya, Koray Günter, Olcan Adın, Tarık Çamdal, Sabri Sarıoğlu, Jason Denayer – Bilal Kısa, Jem Paul Karacan, Yasin Öztekin, Wesley Sneijder, Jose Rodriguez, Emre Çolak – Umut Bulut, Lukas Podolski, Sinan Gümüş