The former Manchester Untied man fired home from a free-kick in the last kick of the game.
The goal was Nani’s second of the game, with the first coming in the 43rd minute. Guilherme equalised for the visitors in the 70th minute setting up an exciting end to the second half.
The win brings Fenerbahce up to the top of the league with seven points in three games. The Yellow Canaries take on Kasimpasa in their next league game after the international break.
Antalyaspor remain in 5th place despite their winning run coming to an end. The Scorpions take host Eskisehirspor in the next round of games.
Luis Nani joined Fenerbahce earlier this summer along with Robin van Persie earlier this summer. Lazar Markovic also joined Fenerbahce from the Premier League today.