Tottenham host the Bundesliga giants at White Hart Lane, kick-off is at 20:05 (GMT).
According to the German outlet Sport 1 the Germany international will sit out the game due to a foot injury.
While the 25-year-old has returned to training following his injury, Dortmund boss Thomas Tuchel isn’t willing to take any chances with this midfield playmakers fitness.
Dortmund recorded a 3-0 at home against Spurs and look set for a comfortable passage to the quarter finals.
Aubamayang opened the scoring for the home side on 30 minutes and Reus scored a second half brace to wrap up a memorable victory for the home side.
The 25-year-old has six assists and three goals in 32 appearances for Dortmund this season.