Markovic has been ruled out with injury and also missed the first leg against the Portuguese side.
Fenerbahce have no other major injury concerns and will have Gokhan Gonul who missed the first-leg with injury available.
The Canaries won the first game 1-0 at the Sukru Saracoglu stadium and are currently second in the Turkish Super League just one point behind leaders Besiktas.
The full Fenerbahce 21-man squad is as follows:
Volkan Demirel, Fabiano Ribeiro, Ertuğrul Taşkıran, Bruno Alves, Simon Kjaer, Michal Kadlec, Abdoulaye Ba, Caner Erkin, Hasan Ali Kaldırım, Gökhan Gönül, Şener Özbayraklı, Mehmet Topal, Josef De Souza, Ozan Tufan, Raul Meireles, Diego Ribas, Volkan Şen, Alper Potuk, Luis Nani, Jose Fernandao, Robin van Persie.